Pre - Preparation: Protein (Polymer)
DaWei needs to obtain the Unfolded Protein Strand.. First he adds urea to the protein. This urea denaturises the protein into a polymer. After doing so he needs to remove the denatured protein. He then makes adds acetic acid at 5%.
Step 1: Taking out the right vial containing his prepared solution.
Step 2: The bag to contain the solution is soaked in deionised water!
Step 3: DaWei proceeds to homogenise the 5% acetic acid in deionised (total 3 Litres!) water using a Homogeniser. The white motion blur you see is a magnetic mixer which is spun by a electromagnet below the machine.
Step 4: DaWei Proceeds to carefully add the protein into the bag.
Step 5: Securing the bag with clips!
Step 6: The bag is placed into a container containing roughly 800 mililitres of 5% acetic acid. This first round will be put overnight. Subsequent ones are done for 2-3 hours to remove any excess urea. Stay tuned!
How this last step works:
The 5% acetic acid is maintained to make sure to make sure the protein stays soluble in the solution. The urea molecules are small enough to pass through the semi-permeable membrane of the bag. This is done repeatedly, removing any remaining urea.
Points to note: Dialysis although share similar steps to osmosis, they are different, to understand do check out a website by clicking here.
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