The dialysis bag containing urea, denatured protein and 5% acetic acid was further processed so that all the urea can removed from the bag. This step was repeated twice, each time roughly 2-3 hours. The result was a bag containing water, 5% acetic acid and the protein, meaning that urea has been successfully removed.
In order to obtain the pure protein powder, the bag was placed in a freezer at -80 degrees Celsius. The bag then turned solid hard. Afterwards, Dawei placed the bag in a freeze dryer. Ice and acetic acid which were initially present soon sublimed. Liquid water and aqueous acetic acid were not formed as both substances were sublimed.
The final product contains the protein only.
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