The Lab:
Step 1: DaWei pours out the 5% acetic acid and removed urea
Step 2: He then replaces it with a fresh solution of 5% acetic acid free of urea!
Step3: Steps 1-2 are repeated once more at 2-3 hours each time.
Step 4: Placing it in rack and puncturing a hole. This is to ensure that the frozen water and acetic acid can be sublimed when in the freeze dryer, leaving only the protein
It it then placed in a free dryer for 1 h and 30 mins to ensure that everything is frozen.
The -80 Freezer!
Step 5: DaWei quickly places the rack containing the vial in a special glass which can withstand pressures close to vacuum. This has to be done quickly to ensure that the solution does not melt!
Done!
This is left overnight to ensure that all water and the 5% acetic is removed, leaving only the protein.
The freeze-dryer machine!
Our attempts successful!:
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